Simultaneous Determination of Indapamide, Amlodipine Besylate and Perindopril Arginine Combined in Tablet Dosage Form Using High Performance Liquid Chromatography

Abstract

determination of a single-pill triple therapy containing: Indapamide, Amlodipine Besylate, and Perindopril. The validation parameters are tested by following the international conference of harmonization (ICH) guidelines. The three components were successfully separated in just 14 minutes. The aforementioned components were well-separated on an Inertsil C8- column (250mm x 4.6mm, 5µm) using a mobile phase prepared by mixing of Triethylamine counter ion solution (pH 3.0) with Acetonitrile at a fixed ratio of 2:1. Analysis was performed at a wavelength of 205 nm, eluent flow rate and column oven temperature was 1.5 ml/min, and 40°C, respectively. Linearity was observed in the concentration ranges of (20-30) µg/mL for Indapamide, and (80-120) µg/mL for both Amlodipine Besylate and Perindopril Arginine. We found the recovery percentages to be 99.98%, 101.04%, and 100.58% for Perindopril Arginine, Amlodipine Besylate, and Indapamide, respectively. Further, we found the detection limits to be 0.38 µg/mL, 0.99 µg/mL and 3.65 µg/mL, and the obtained quantitation limits were 1.16 µg/mL 3.01 µg/mL, and 11.06 µg/mL for Indapamide, Amlodipine Besylate, and Perindopril Arginine, respectively.