IN VITRO STUDIES ON CALLUS INDUCTION OF MORINGA PEREGRINA (FORSSK) FIORI AND ANTIFUNGAL ACTIVITY OF PLANT EXTRACTS

Authors

  • Linda Alrayes
  • Mohamad Shatnawi
  • Wesam Al Khateeb

Abstract

In this study satisfactory method for callus induction and growth has been developed for Moringa peregrina. Maximum callus size was observed on MS medium supplemented with 1.0 mg/L 2,4-D, while maximum callus weight was achieved on MS medium supplemented with 2.0 mg/L 2,4-D. Both in vitro, callus and ex vito dried leaf extract showed similar antimicrobial activity. Antifungal activity of aqueous, methanolic, ethanolic, and acetonic extracts of both in vitro plantlets and ex vitro plant were evaluated by the agar well-diffusion method against Aspergillus brasiliensis, Aspergillus italicium, Aspergillus niger brown (mutant), Aspergillus niger black, Penicillium digilatum, Phytophthora infestans and Candida albicans. In vitro aqueous extract was found to be more effective compared to the positive control and the ex vitro aqueous extract against Pencillium digilatum and Phytophthora infestans, while in vitro methanolic extract was more effective than positive control against Candida albicans.

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Published

2016-12-19

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Articles