Micropropagation of Sweet Cherry Dwarf Rootstock (PHL-C)

Abstract

PHL-C is one of dwarf sweet cherry rootstocks which is a hybrid between Prunus.avium L. × Prunus.cerasus L. and is recommended for high density plantation of the commercial orchards. In this study the effect of different media types and plant growth regulators for proliferation and rooting of PHL-C rootstock were investigated in Khorasan Razavi Agriculture and Natural Resources Research and Education Center. For proliferation, three culture media (MS, DKW, WPM) and four concentrations of BAP (0.0, 1.0, 1.5, 2.0 mgL-1) were experimented. Also, effects of six culture media (solid and double phase of MS, DKW, WPM) supplemented with four concentrations of IBA (0, 1, 1.5 and 2 mgL-1) on rooting were investigated. Results showed that, the highest shoot multiplication rate was observed in MS + 1 BAP mgL-1, while the highest quality (strong and vigorous Explants, with no signs of vitrification, necrosis, yellow apex and leaves ( of plantlet was observed in DKW medium. Moreover, MS media supplemented with either 1.5 or 2 mgL-1 IBA proved to be superior to other treatments in terms of root number and length.