Rescuing Endangered Moringa peregrina (frossk) fiori by Cryopreservation Using Vitrification and Encapsulation- Vitrification Protocols

Abstract

Cryopreservation was applied to assess the possibility of using long-term conservation of Moringa peregrina (frossk) fiori using vitrification and encapsulation-vitrification techniques. In addition, the genetic stability of the recovered shoot tips after cryogenic storage was assessed using Amplified Fragment Length Polymorphism (AFLP) technique. Data obtained from the vitrification experiments revealed that using the 0.4 M sucrose plus 2 M glycerol as a loading solution yields the highest recovery rate (20%) compared to the other loading solutions cocktails. In another vitrification experiment, the maximum survival and recovery rates, i.e. 75% and 35% respectively were obtained in a combination of loading and vitrification solutions that consisted of 10% DMSO + 0.75 M sucrose and PVS2. In the encapsulation-vitrification experiment, cryopreserved M. peregrina shoot tips recorded the highest survival and recovery rates, i.e. 75% and 20%, respectively. Moreover, no genetic variation was found between the control samples and preserved shoot tips.